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2. | | KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377. Biblioteca(s): Embrapa Meio Ambiente. |
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3. | | MENDES, R.; KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L. Metagenomic analysis of sheep rumen microbiome for carbohidrate-active genes discovery. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 16., 2016, Montreal. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2016. p. 836. Biblioteca(s): Embrapa Meio Ambiente. |
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4. | | LOPES, L. D.; LIMA, A. O. S.; SILVA, L. R. F.; ROMAGNOLI, E. M.; TAKETANI, R. G.; FERREIRA, C; ABDALLA, A. L.; MENDES, R. Identificação de enzimas lignocelulolíticas no microbioma do rúmen de ovinos usando metagenômica shotgun. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 901-1 Biblioteca(s): Embrapa Meio Ambiente. |
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5. | | LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen microbiome for carbohydrate-active enzymes. Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015. Biblioteca(s): Embrapa Meio Ambiente. |
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6. | | SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D. Endo- and exoglucanase activities in bacteria from mangrove sediment. Brazilian Journal of Microbiology, Piracicaba, v. 44, n. 3, p. 969-976, 2013. Biblioteca(s): Embrapa Meio Ambiente. |
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7. | | LOPES, L. D.; SILVA, L. R. F.; ROMAGNOLI, E. M.; FERREIRA, C; TAKETANI, R. G.; LOUVANDINI, H; LIMA, A. O. S.; ABDALLA, A. L.; MENDES, R. Sheep rumen microbiome sequencing using Ion Torrent (PGM) platform. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 12., 2013, Ljubljana (Slovenia). Networking and plasticity of microbial communities: the secret to success. Ljubljana: University of Ljubljana. 2013. p. 68, ref. P31. Biblioteca(s): Embrapa Meio Ambiente. |
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8. | | CAVALETT, A.; SILVA, M. A. C. da; TOYOFUKU, T.; MENDES, R.; TAKETANI, R. G.; PEDRINI, J.; FREITAS, R. C. de; SUMIDA, P. Y. G.; YAMANAKA, T.; NAGANO, Y.; PELLIZARI, V. H.; ALVAREZ PEREZ, J. A. LIMA, A. O. S.; KITAZATO, H.; LIMA, A. O. de S. Dominance of Epsilonproteobacteria associated with a whale fall at a 4204 m depth - South Atlantic Ocean. Deep Sea Research Part II, v. 146, p. 53-58, 2017. Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 8 | |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
04/01/2016 |
Data da última atualização: |
10/03/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. |
Afiliação: |
LUCAS DANTAS LOPES; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; RODRIGO GOUVEA TAKETANI; PHILLIP DARIAS, UNIVALI; LILIA RAQUEL FE DA SILVA, UFPI; EMILIANA MANESCO ROMAGNOLI; HELDER LOUVANDINI, CENA-USP; ADIBE LUIZ ABDALLA, CENA-USP; RODRIGO MENDES, CNPMA. |
Título: |
Exploring the sheep rumen microbiome for carbohydrate-active enzymes. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes MenosAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) represen... Mostrar Tudo |
Palavras-Chave: |
Ion torrent (PGM). |
Thesagro: |
Amilase; Bactéria; Enzima; Ovelha; Rúmen. |
Thesaurus NAL: |
Amylases; Bacterial communities; Lignocellulose; Metagenomics; Phylogeny. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02652naa a2200349 a 4500 001 2032734 005 2016-03-10 008 2015 bl uuuu u00u1 u #d 100 1 $aLOPES, L. D. 245 $aExploring the sheep rumen microbiome for carbohydrate-active enzymes.$h[electronic resource] 260 $c2015 520 $aAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes 650 $aAmylases 650 $aBacterial communities 650 $aLignocellulose 650 $aMetagenomics 650 $aPhylogeny 650 $aAmilase 650 $aBactéria 650 $aEnzima 650 $aOvelha 650 $aRúmen 653 $aIon torrent (PGM) 700 1 $aLIMA, A. O. S. 700 1 $aTAKETANI, R. G. 700 1 $aDARIAS, P. 700 1 $aSILVA, L. R. F. 700 1 $aROMAGNOLI, E. M. 700 1 $aLOUVANDINI, H. 700 1 $aABDALLA, A. L. 700 1 $aMENDES, R. 773 $tAntonie van Leeuwenhoek, Amsterdam$gv. 108, n. 1, p. 15-30, 2015.
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